Figure 3.

Establishing a statistical threshold for relatedness distribution. (A) Benchmarking putative cross-reactivity pairs in allele agnostic simulation. In total, 5 million peptide pairs were simulated and categorized based on relatedness intervals. For each pairwise comparison was calculated an empirical p-value. The left-sided y-axis shows the percentage of cases per bin (i.e., intervals). The blue line represents the average p-value in each interval (second y-axis). Additionally, red and brown are associated with standard p-value thresholds, 0.05 and 0.01, respectively. (B) Table covering experimentally validated CR cases. MAGEA3-TITIN was retrieved at 14-16 bin, which holds less than 0.1% of putative cases.