Table 3.
Distribution of positive cases by felid species, melting temperatures, and sequencing.
| Felids | n/tot (%) | Tm (°C) | Real-time PCR | n/tot | Sequencing | % Identity |
|---|---|---|---|---|---|---|
| Domestic cats | 31/206 (15) | 78/78.5 | Hepatozoon spp. | 12/31 19/31 |
H. felis
H. silvestris |
99.7–100% 97–100% |
| 6/206 (2.9) | 81 | Cytauxzoon spp. | 6/6 | C. europaeus | 100% | |
| Wildcats | 8/19 (42.1) | 78/78.5 | Hepatozoon spp. | 6/8 2/8 |
H. felis
H. silvestris |
97.3–99.7% 99.7% |
| 4/19 (21) | 81 | Cytauxzoon spp. | 4/4 | C. europaeus | 98.8–100% | |
| Exotic felids | 2/12 (16.7) | 78/78.5 | Hepatozoon spp. | 1/2 1/2 |
H. felis
H. silvestris |
99.7% 99.7% |