Table 3.
Phenotypic characteristics that distinguish between strain ATCHAT, ATCH28T, and type strains of closely related species of the genus Halomonas.
| Characteristic | ATCHAT | ATCH28T | H. salicampi a | H. vilamensis b | H. ventosae c | H. salina d |
|---|---|---|---|---|---|---|
| Cell shape | Rod | Short rod | Rod | Rod | Short rod | Short rod |
| Cell size (μm) | 3.6–6.0 × 0.8 | 2.0–3.0 × 1.0 | 1.1–1.3 × 0.3–0.5 | 2.0–4.0 × 1.0–1.6 | 1.2–1.5 × 0.7–0.8 | 2.0–2.5 × 0.7–0.8 |
| Pigmentation | Cream | Cream | Cream | Cream-pink | Cream | yellowish-cream |
| Flagellum | Lopho | Mono | Lopho | Lopho | ND | Nonmotile |
| NaCl range (% w/v) | 3–20 | 0.5–17 | 0–23 | 1–25 | 3–15 | 2.5–20 |
| NaCl optimum (% w/v) | 10 | 10 | 14 | 5–10 | 6–9 | 5 |
| Temperature range (°C) | 4–40 | 4–40 | 10–55 | 5–40 | 15–50 | 15–40 |
| Temperature optimum (°C) | 30 | 30 | 28 | 30 | ND | 32 |
| pH range | 6.0–9.5 | 6.0–9.0 | 7.0–10.8 | 5.0–10.0 | 6.0–10.0 | 6.0–10.0 |
| pH optimum | 7.5 | 8.5 | 8.5 | 7.0–8.0 | ND | 7.2 |
| DNA G + C content (mol%) | 55.7 | 66.5 | 54.7 | 55.0 | 72.6–74·6 | 60.7–64.2 |
| Nitrate reduction | − | + | + | + | + | + |
| Production of H2S | − | − | − | − | + | − |
| Hydrolysis of: | ||||||
| Aesculin | + | + | − | − | − | − |
| Gelatin | − | − | − | + | − | − |
| DNA | − | − | − | − | − | − |
| Utilization of: | ||||||
| Glycerol | − | − | + | − | + | ND |
| D-Galactose | − | + | − | − | + | − |
| D-Glucose | − | + | + | − | + | − |
| D-Mannose | − | − | − | − | ND | + |
| D-Sorbitol | − | − | − | − | + | + |
| D-Trehalose | − | + | + | − | ND | + |
| Maltose | − | + | + | − | + | + |
| Sucrose | − | + | + | − | ND | + |
| Turanose | − | + | − | − | ND | ND |
| Acetic acid | + | + | ND | + | ND | + |
| Citric acid | + | − | − | − | + | + |
| Lactic acid | − | − | ND | − | + | + |
| Malic acid | − | + | ND | + | + | + |
| Propionic acid | + | + | ND | − | + | − |
| Gluconic acid | − | + | − | − | + | + |
| L-Alanine | + | + | ND | + | − | + |
| L-Serine | − | + | ND | − | ND | − |
+, positive; −, negative; ND, no data available.