REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken polyclonal anti-Anillin	Hickson lab El amine et al. (2019)18	N/A
Mouse monoclonal anti-α-tubulin (clone DM1A)	Sigma-Aldrich	Cat#CP06, RRID:AB_2617116
Rabbit Anti-Green Fluorescent Protein (GFP) Polyclonal Antibody	Molecular Probes (ThermoFisher Scientific)	Cat#A-6455, RRID:AB_221570
Mouse Anti-Drosophila Peanut Monoclonal Antibody, Unconjugated	Developmental studies hybridoma bank	Cat#4C9H4 anti-peanut, RRID:AB_528429
Mouse Anti-Drosophila Rho1 Monoclonal Antibody, Unconjugated	Developmental studies hybridoma bank	Cat# p1D9 (anti-rho1), RRID:AB_528263
Bacterial and virus strains
Escherichia coli NEB® 5-alpha Chemically competent cells	New England Biolabs	Cat# C2987I
Escherichia coli BL21(DE3) Chemically competent cells	Sigma-Aldrich	Cat# CMC0014
Escherichia coli One Shot™ TOP10 Chemically competent cells	ThermoFisher Scientific	Cat# C404010
Chemicals, peptides, recombinant proteins
Alexa Fluor® 647 Phalloidin antibody	Thermo Fisher Scientific	Cat#A22287, RRID:AB_2620155
Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat#A-11001, RRID:AB_2534069
Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	Thermo Fisher Scientific	Cat#A-21235, RRID:AB_2535804
Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546	Thermo Fisher Scientific	Cat#A-11003, RRID:AB_2534071
Aurora alpaca secondary antibody anti-chicken IgY ATTO 565 conjugated	Immune Biosolutions	Cat#Y00008-565, RRID:AB_2622026
Aurora alpaca secondary antibody anti-chicken IgY	Immune Biosolutions	Cat#Y00008-002, RRID:AB_2622024
FugeneHD transfection reagent	Promega corporation	Cat#E2311
Lipofectamine 2000	ThermoFisher Scientific	Cat#11668030
CellfectinII	ThermoFisher Scientific	Cat#10362100
Latrunculin A	Sigma-Aldrich	Cat#428021
Puromycin dihydrochloride	Sigma-Aldrich	Cat#P8833
Fluoromount-G	SouthernBiotech	Cat# 0100-01
Opti-MEM	Thermo Fisher Scientific	Cat#31985062
Fetal Bovine Serum, qualified, heat inactivated, United States	Thermo Fisher Scientific	Cat#16140071
Schneider’s Drosophila medium	Thermo Fisher Scientific	Cat#21720024
Dulbecco’s modified Eagle’s medium DMEM, high glucose, pyruvate	Thermo Fisher Scientific	Cat#11995040
CO2 independent medium	Thermo Fisher Scientific	Cat#18045088
Clarity Max™ Western ECL Substrate, 100 mL	Bio-Rad	Cat#1705062
Critical commercial assays
pENTR™/D-TOPO™ Cloning Kit	Thermo Fisher Scientific	Cat#K240020
T7 RiboMAX™ Express Large Scale RNA Production System	Promega corporation	Cat#P1320
Gateway LR clonase enzyme mix	Thermo Fisher Scientific	Cat#11791019
T4 DNA Ligase	Thermo Fisher Scientific	Cat#15224025
Experimental models: Cell lines
D.melanogaster Schneider’s S2 cells	University of California San Francisco	N/A
HeLa cell line	ATCC	Cat#CRM-CCL-2, RRID:CVCL_0030
S2 cells expressing Anillin-GFP and mCherry-tubulin	This paper	N/A
S2 cells expressing Anillin-RBD∗-GFP and mCherry-tubulin	This paper	N/A
Oligonucleotides
siRNA targeting sequence: pGL2 Firefly Luciferase(CGUACGCGGAAUACUUCGA)	Eurogentec	Cat#SR-CL010-005
siRNA targeting sequence: hs ANLN (CGAUGCCUCUUUGAAUAAA)	Dharmacon	N/A
Primers used for all constructs cloned, see Table S1	This paper	N/A
Recombinant DNA
pCoPuro plasmid	Addgene	RRID:Addgene_17533
D.mel pENTR-Anillin-WT-ds resistant, pMT-Anillin-ds resistant-WG/WCh	This paper	N/A
D.mel pENTR-Anillin-RBD∗-ds resistant, pMT-Anillin-RBD∗-WG/WCh	This paper	N/A
D.mel pMT-Anillin-ΔC-term-WCh	Kechad et al.38	N/A
D.mel pENTR-Anillin-ΔN-term-ds resistant, pMT-Anillin-ΔN-term-WCh	This paper	N/A
D.mel pENTR-Anillin-ΔN-term-RBD∗-ds resistant, pMT-Anillin-ΔN-term-RBD∗-WCh	This paper	N/A
D.mel pENTR-Anillin-AHD-ds resistant, pMT-Anillin-AHD-WCh	This paper	N/A
D.mel pENTR-Anillin-PHD-ds resistant, pMT-Anillin-AHD-WCh	This paper	N/A
D.mel pMT-Anillin-ΔAHD-WCh	Laboratory of Gilles Hickson	N/A
D.mel pENTR-Anillin-Δ796-1050-ds resistant, pMT-Anillin-Δ796-1050-WCh	This paper	N/A
D.mel pENTR-Anillin-ΔRBD(Δ850-915)-ds resistant, pMT-Anillin-ΔRBD(Δ850-915)-WCh	This paper	N/A
D.mel pENTR-Anillin-Δ796-928-ds resistant, pMT-Anillin-Δ796-928-WCh	This paper	N/A
D.mel pENTR-Anillin-PH+54-(1051-1239)-ds resistant, pMT-Anillin-PH+54 (1051-1239)-WCh	This paper	N/A
D.mel pENTR-Anillin-PH+103-(998-1239)-ds resistant, pMT-Anillin-PH+103 (998-1239)-WCh	This paper	N/A
D.mel pENTR-Anillin-PH+206-(899-1239)-ds resistant, pMT-Anillin-PH+206 (899-1239)-WCh	This paper	N/A
D.mel pENTR-Anillin-ΔPHD-ds resistant, pMT-Anillin-ΔPHD-WCh	This paper	N/A
D.mel pMT-Septin1-WG	Laboratory of Gilles Hickson	N/A
D.mel pMT-Septin2-WG and pMT-Septin2-WCh	Laboratory of Gilles Hickson	N/A
Hs pEGFP-Anillin-WT-RNAi resistant-C1	Piekny & Glotzer10 Addgene Note this is the shorter 1087 amino acid isoform of the two isoforms of human ANLN	RRID:Addgene_68027
Hs pEGFP-Anillin-A703E-RNAi resistant-C1	Kind gift from Dr Alisa Piekny, Concordia University, Montreal, Canada. Note that this is the shorter 1087 amino acid isoform of the two isoforms of human ANLN. A703 corresponds to A740 of the longer 1124 amino acid isoform described in Sun et al.23	N/A
pEGFP-N1-SEPT2 (mouse)	Kind gift from Dr Elias Spiliotis, Drexel University, Philadelphia, USA	N/A
pEGFP-C3-SEPT6 (Human)	Kind gift from Dr Elias Spiliotis, Drexel University, Philadelphia, USA, Kinoshita et al.35	N/A
pEGFP-SEPT7 (Rat)	Kind gift from Dr Elias Spiliotis, Drexel University, Philadelphia, USA	N/A
pmCherry-N1-SEPT9 (human isoform 1)	Kind gift from Dr Elias Spiliotis, Drexel University, Philadelphia, USA Dolat et al., 201463	N/A
Software and algorithms
Volocity version 6.3	Perkin Elmer http://www.perkinelmer.com/pages/020/cellularimaging/products/volocity.xhtml	RRID:SCR_002668
GraphPad Prism 7	GraphPad http://www.graphpad.com/	RRID:SCR_002798
Adobe photoshop CS6	Adobe https://www.adobe.com/products/photoshop.html	RRID:SCR_014199
Adobe illustrator CS6	Adobe http://www.adobe.com/products/illustrator.html	RRID:SCR_010279