REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-AKT	Cell Signaling	#9272
Anti-p-AKT (S473)	Cell Signaling	#9271
Anti-p-AKT (T308)	Cell Signaling	#13038
Anti-ERK1/2	Cell Signaling	#4695
Anti-p-ERK1/2	Cell Signaling	#4370
Anti-p38	Cell Signaling	#8690
Anti-p-p38	Cell Signaling	#4511
Anti-JNK	Cell Signaling	#9252
Anti-p-JNK	Cell Signaling	#4668
Anti-p-AMPKα (T172)	Cell Signaling	#50081
Anti-AMPKα	Cell Signaling	#5832
Anti- GSK3β	Cell Signaling	#9315
Anti-p-GSK3β (S9)	Cell Signaling	#9323
Anti-GAPDH	Cell Signaling	#2118
Biotinylated anti-PIP3 IgM	Echelon	#Z-B345
Anti-P2Y2R	Abclonal	#A13923
Anti-VNUT	Proteintech	#26731-1-AP
Rabbit anti-mouse lgG (light chain specific) secondary antibody, HRP	Cell Signaling	#58802
Goat anti-rabbit lgG (H+L) secondary antibody, HRP	ThermoFisher Scientific	#31460
Bacterial and virus strains
iATPSnFR1.1	Lobas et al.41	Addgene #102549
Chemicals, peptides, and recombinant proteins
EMEM	Lonza	12-611Q
Opt. MEM	Gibco	11058021
Depleted DMEM	Lonza	A1443001
Fetal bovine serum	ThermoFisher Scientific	A5256701
DMSO	EMD Milipore	102952
ATP disodium salt	MilliporeSigma	A1852
UTP disodium salt	MilliporeSigma	94370
UDP disodium salt	Tocris	3111
ATPγS	Jena Bioscience	NU-406
UTPγS	Jena Bioscience	NU-416
ADPβS	Jena Bioscience	NU-433
NF546	Tocris	3892/10
ARC-118925XX	Tocris	4890/5
ARL67156 trisodium salt	Tocris	1283/10
A23187, free acid	Tocris	1234/10
Metformin	Enzo Life Sciences	50-200-8556
Phenformin HCl	BioVision	1889
Buformin HCl	Cayman Chemical	18507
Proguanil HCl	MilliporeSigma	G7048
Bafilomycin A1	Enzo	BML-CM110-0100
Allantoin	Cayman Chemical	30204
PBS (10x)	VWR	J373-4L
SYBR Green PCR master mix	Applied Biosystems	4309155
4X Laemmli Sample Buffer	Bio-Rad	1610747
TG-SDS (10x)	VWR	0783-5L
TBS (10x)	ThermoFisher Scientific	J60764-k7
TAE (10x)	VWR	J63677
Tween 20	VWR	M147-1L
DharmaFECT-1	Dharmacon / Horizon Discovery	T-2001-03
G418	EMD Milipore	345812
Hematoxylin	VWR	517-28-2
Eosin	VWR	10863-274
Bluing Reagent	Richard-Allan Scientific	7301
U73122	Tocris	1268
Monensin sodium salt	Enzo Life Sciences	ALX-380-026-G001
D-Glucose	VWR	AAJ60067-AK
Mannitol	VWR	A14030
LysoView 633	Biotium	70058-T
MemGlow640	MemBright	MG04
MemGlow488	MemBright	MG01
Mini-protean TGX gels	Bio-Rad	4561033
MANT-ATP	ThermoFisher Scientific	M12417
Insulin (Humulin® U-100)	Eli Lilly	NDC 54868-3619-0
HEPES	MilliporeSigma	H4034
Critical commercial assays
Fluorforte Calcium Mobilization Assay	Enzo Life Science	ENZ-51016
ENLIGHTEN ATP Assay	Promega	FF2000
Colorimetric/fluorometric ATP Assay	Abcam	ab83355
Amplex Red Glucose Assay	Invitrogen	A12222
Glycogen Detection Assay	Cayman Chemical	700480
Glucose Uptake Assay	Abcam	ab136956
ADP Assay Kit	Sigma-Aldrich	MAK133
RNeasy Mini Kit	Qiagen	74104
Experimental models: Cell lines
HepG2 cells	ATCC	HB-8065
Primary Human Hepatocytes	Applied Biological Materials Inc.	T0051
Experimental models: Organisms/strains
B6.129P2-P2ry2 tm1Bhk /J (mice)	Jackson Laboratory	009132
C57BL/6NJ (mice)	Jackson Laboratory	005304
Oligonucleotides
ON-TARGET plus SMART Pool L-003688-00-0005 (human P2RY2, NM_002564)	Dharmacon	L-003688-00-0050
ON-TARGET plus Non-targeting Control Pool	Dharmacon	D-001810-10-20
Software and algorithms
Prism 9	GraphPad	https://www.graphpad.com/
ImageJ	NIH	https://imagej.net/ij/index.html
Excel	Microsoft Office	https://www.microsoft.com/en-us/microsoft-365
BioRender	Online	https://biorender.com/
Other
True Metrix Go® glucometer	Trividia Health	KRKY0184203431
VectaMount® AQ Aqueous Mounting Medium	Vector® Laboratories	H-1800
Standard chow diet	ENVIGO	TD.08485
High-fat diet	ENVIGO	TD.88137
AMG EVOS digital inverted microscopy	ThermoFisher Scientific	AMEX1000