REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human APJ Antibody	R&D	Cat# MAB8561; RRID: AB_2058383
Anti-CD31	Abcam	Cat# ab32457; RRID: AB_726369
Anti-CD34	Abcam	Cat# ab81289; RRID: AB_1640331
Anti-CD43	Abcam	Cat# ab101533; RRID: AB_10710687
Alexa Fluor 488	Invitrogen	Cat# A11034; RRID: AB_2576217
Alexa Fluor 488	Life Technologies	Cat# A21202; RRID: AB_141607
Alexa Fluor 594	Life Technologies	Cat# A-21207; RRID: AB_141637
Anti-SETD7	Abcam	Cat# ab124708; RRID: AB_10975432
Anti-β-Catenin	CST	Cat# 9562s; RRID: AB_331149
Anti-GAPDH	Proteintech	Cat# 60004-1-1g; RRID: AB_2107436
Anti-Histone H3 (mono methyl K4)	Abcam	Cat# ab8895; RRID: AB_306847
Anti-POU5F1	CST	Cat# 2750S; RRID: AB_823583
Anti-NANOG	CST	Cat# 3580S; RRID: AB_2150399
Anti-SOX2	CST	Cat# 3579S; RRID: AB_2195767
Phospho-β-Catenin	CST	Cat# 4176T; RRID: AB_1903923
HRP-conjugated AffiniPure Goat Anti-mouse IgG	Proteintech	Cat# SA00001-1; RRID: AB_2722565
Anti-rabbit IgG HRP-linked Antibody	CST	Cat# 7074P2; RRID: AB_2099233
Lamin B1	Proteintech	Cat# 66095-1; RRID: AB_11232208
Human APJ-APC-conjugated Antibody	R&D	Cat# FAB8561A
Anti-FLAG	Sigma	Cat# F1804; RRID: AB_262044
PE Mouse Anti-Human CD31	BD Biosciences	Cat# 555446; RRID: AB_395839
APC Mouse Anti-Human CD34	BD Biosciences	Cat# 55582; RRID: AB_2228972
Mouse IgG3 APC-conjugated Antibody	R&D	Cat# 1C007A
PE Mouse IgG1, κ Isotype Control	BD Biosciences	Cat# 559320; RRID: AB_397218
propidium iodide (PI)	Solarbio	Cat# C0080
DAPI	Solarbio	Cat# C0060
APC Mouse Anti-Human CD43	BD Biosciences	Cat# 560198; RRID: AB_1645460
Deposited data
Raw and analyzed data	This paper	GEO: GSE228287
Critical commercial assays
EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix	transgene	Cat# N10613
Hieff UNICON® Universal Blue qPCR SYBR Green Master Mix	YESEN	Cat# 11184ES03
Nuclear and Cytoplasmic Protein Extraction Kit	Beyotime	Cat# P0028
EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix	transgene	Cat# N10613
apoptosis detection kit	BD Biosciences	Cat# 556547
protein G superparamagnetic beads	Invitrogen	Cat# 10003D
Lipofectamine Stem regent	Invitrogen	Cat# STEM00001
Experimental models: Cell lines
hESC: H1	WiCell	RRID: CVCL_9771
SETD7 KO H1	This paper	N/A
SETD7 KD H1	This paper	N/A
SETD7 OE H1	This paper	N/A
b-catenin OE H1	This paper	N/A
b-catenin K180R OE H1	This paper	N/A
Scramble control H1	This paper	N/A
Empty Vector control H1	This paper	N/A
Oligonucleotides
RT-qPCR primers	This paper	Table S4
SETD7 gRNA1	This paper	Table S2
SETD7 gRNA2	This paper	Table S2
SETD7 inducible OE primers	This paper	Table S1
SETD7 inducible KD primers	This paper	Table S3
inducible KD Scramble primers	This paper	Table S3
CTNNB1 inducible OE primers	This paper	Table S1
CTNNB1 inducible K180R primers	This paper	Table S1
Software and algorithms
FlowJo	FlowJo	https://www.flowjo.com
GSEA v4.0	Subramanian et al.71	http://software.broadinstitute.org/gsea/index.jsp
HISAT2-StringTie	Pertea et al.72	http://ccb.jhu.edu/software.shtml
R v3.6	R-Project	https://www.r-project.org/
GraphPad Prism	GraphPad	https://graphpad.com
Chemicals, peptides, and recombinant proteins
Activin A	PeproTech	Cat# 120-14-1000
BMP4	PeproTech	Cat# 120-05ET-1000
bFGF	PeproTech	Cat# 100-18B-100
VEGF	PeproTech	Cat# 100-20-100
TPO	PeproTech	Cat# 30018
EPO	Prospec	Cat# 10064
SCF	Prospec	Cat# CYT-255
IL11	PeproTech	Cat# 200-11
IL6	STEMCELL Technologies	Cat# 78050.1
IL3	Sigma	Cat# I1646
GM-CSF	PeproTech	Cat# HZ-1002
GCSF	PeproTech	Cat# HZ-1207
Flt-3L	PeproTech	Cat# 300-19
Y27632	Calbiochem	Cat# 688000
hydrocortisone	Sigma	Cat# H0888
L-ascorbic acid	Sigma	Cat# A8960-5G
heparin	Sigma	Cat# H3149-500KU-9
mTeSR+supplement	Stem Cell	Cat# 85852
DMEM/F-12(1;1) basic	Gibco	Cat# C11330500BT
Fetal bovine serum	Gibco	Cat# 16000-044
Penicillin-Streptomycin	Gibco	Cat# 15140-122
Accutase	Gibco	Cat# AB11105-01
Costom TESR+supplement	Stem Cell	Cat# 05892