Skip to main content
. 2023 Feb 9;43(7):1060–1076. doi: 10.1177/0271678X231156981

Figure 4.

Figure 4.

Long-term selenium supplementation (6 weeks) following tMCAO prevents secondary neural degeneration and promotes neurogenesis in the infarct hemisphere. (a) NeuN (green) and PV (red) immunostaining of tMCAO mice or (b) tMCAO + Se mice 6 weeks after tMCAO. The infarct was in the right hemisphere. The red arrow indicates secondary hippocampal cell death, the yellow arrow indicates PV-positive neurons in the thalamic reticular nucleus (TRN), and the white arrow indicates a loss of intact cortical area due to the stroke insult. (c) c-Fos (red) and NeuN (green) immunostaining of the infarct DG for tMCAO or (d) tMCAO + Se mice 60 min after the APA retrieval test. (e) DCX (red) immunostaining of the infarct DG for tMCAO or (f) tMCAO + Se mice 6 weeks after tMCAO. (g) Relative NeuN fluorescence intensity in the CA1 region of the right hippocampus. (h) The number of c-Fos-positive cells per right DG following APA retrieval. (i) Relative PV fluorescence intensity in the TRN area of the right hemisphere. (j) The number of DCX-positive immature neurons per right DG. (g–j: unpaired two-tailed t-test, n = 5–10 mice per group). Data represent mean ± SD.