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. 2023 May 23;26(6):106960. doi: 10.1016/j.isci.2023.106960

Figure 4.

Figure 4

Gavage of Western diet-fed Apoe−/− mice with live bacteria changes the levels of plasma metabolites

(A) Principal component analysis of plasma metabolite profiles in mice treated with vehicle or live bacteria.

(B and D–F) Heatmap of differentially affected metabolites screened by OPLS-DA. The grids filled with graduated color indicate the relative abundance of metabolites, and the grids filled with pure red and blue represent the correlation between metabolites and lipids. Differential metabolites were defined by a p value smaller than 0.05 and a VIP value larger than 1. Two-tailed Student’s t test was used for comparison between two groups.

(C) Boxplots of LCA and HMB in mice treated with vehicle or bacteria.

(G) The scatterplots of linear regression of LCA with TG, LDLC, and CHOL. WT, Western diet-fed normal C57BL/6J mice treated with vehicle (n = 7); Apoe−/− control, Western diet-fed Apoe−/− mice treated with vehicle (n = 6); B, Western diet-fed Apoe−/− mice treated with B. cellulosilyticus (n = 7); F, Western diet-fed Apoe−/− mice treated with F. longum (n = 8); R, Western diet-fed Apoe−/− mice treated with R. intestinalis (n = 7); B,F,R, Western diet-fed Apoe−/− mice treated with the cocktail of the three bacteria (n = 5).