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. 2023 May 26;26(6):106985. doi: 10.1016/j.isci.2023.106985

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© 2023 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Differential organizations of axonal projections of DgI-L5 subpopulations to the CeA

(A) Illustrations depicting AAV injections for Cre-induced GFP expression in the L5a population. Note that a retrograde AAV carrying Cre was injected into the CeA contralateral to the insula injected with an AAV carrying Cre-inducible GFP.

(B) Fluorescent images of the AAV-injected site in the insula. Left: mCherry (a Cre expression reporter), middle: GFP, right: merged (also counterstained with DAPI, blue).

(C) Fluorescent images of the AAV-injected site in the CeA. Left: mCherry (indicative of the AAV injection), middle: GFP (axon arbors restricted to the CeL/CeC, inset), right: merged (also with DAPI).

(D) GFP-expressing axon arbors in the amygdala ipsilateral to the insula injected with the AAV vector.

(E) A higher magnification view of the CeA ipsilateral to the insula with the AAV injection. Left: GFP (axon arbors restricted to the CeL/CeC), middle: anti-RGS14 immunostaining identifying the CeL/CeC but not CeM, right: merged.

(F) Illustrations depicting AAV injections for Cre-induced GFP expression in the L5b population.

(G) Fluorescent images of the AAV-injected site in the insula. Left: mCherry (Cre), middle: GFP, right: merged (also with DAPI).

(H) Fluorescent images of the AAV-injected site in the ipsilateral hypothalamus. Left: mCherry (AAV injected site), middle: GFP (axon arbors restricted to the PSTh), right: merged (also with DAPI).

(I) GFP-expressing axon arbors in the amygdala ipsilateral to the insula with the AAV injection. Labeled axons are found in the CeA and BMA.

(J) A higher magnification view of the ipsilateral CeA. Left: GFP (axon arbors predominantly located in the CeM), middle: anti-RGS14 immunostaining, right: merged. Scale bars: 0.2 mm (B, E, G and J), 0.5 mm (C, D, H and I), 0.1 mm (inset in C).

(K) Schematic diagram of the summary for CeA projections of DgI-L5 neuronal subpopulations. Abbreviations: 3V, third ventricle; BLA, basolateral amygdala; BMA, basomedial amygdala; cp, cerebral peduncle; LH, lateral hypothalamus; LV, lateral ventricle; MeA, medial amygdala; Pir, piriform cortex; STh, subthalamic nucleus. See also Figure S4.