Table 1.

Primers and PCR conditions used in this study

Primer	Target size (bp)	Sequences (5´-3´)	Reaction conditions	References
hblA F hblA R	1154	AAGCAATGGAATACAATGGG AGAATCTAAATCATGCCACTGC	94°C, 120 s → (94°C, 1 min → 56°C, 1 min → 72°C, 2 min) 35 cycles → 72°C, 300 s	Kim et al. (2011)
hblC F hblC R	740	GATACCAATGTGGCAACTGC TTGAGACTGCTCGCTAGTTG	94°C, 120 s → (94°C, 1 min → 58°C, 1 min → 72°C, 2 min) 35 cycles → 72°C, 300 s	Kim et al. (2011)
hblD F hblD R	829	ACCGGTAACACTATTCATGC GAGTCCATATGCTTAGATGC	94°C, 120 s → (94°C, 1 min → 58°C, 1 min → 72°C, 2 min) 35 cycles → 72°C, 300 s	Kim et al. (2011)
nheA F nheA R	499	TACGCTAAGGAGGGGCA GTTTTTATTGCTTCATCGGCT	94°C, 120 s → (94°C, 1 min → 56°C, 1 min → 72°C, 2 min) 35 cycles → 72°C, 300 s	Kim et al. (2011)
nheB F nheB R	769	CTATCAGCACTTATGGCAG ACTCCTAGCGGTGTTCC	94°C, 120 s → (94°C, 1 min → 54°C, 1 min → 72°C, 2 min) 35 cycles → 72°C, 300 s	Kim et al. (2011)
nheC F nheC R	581	CGGTAGTGATTGCTGGG CAGCATTCGTACTTGCCAA	94°C, 120 s → (94°C, 1 min → 58°C, 1 min → 72°C, 2 min) 35 cycles → 72°C, 300 s	Kim et al. (2011)