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. 2023 Jun 21;15:17590914231183272. doi: 10.1177/17590914231183272

Figure 1.

Figure 1.

(A) Representative images of the immunodetection of the Müller cells markers Glutamine Synthetase (GS) (purple) and CRALBP (green) in mouse primary Müller control cell cultures (A) or after treatment with 100 μM NMDA for 4 h. The actin cytoskeleton was labeled with rhodamine phalloidin (red) and cell nuclei counterstained with 4,6-diamino-2-phenylindole (DAPI) in blue. (B) Representative images of the immunodetection of GFAP CRALBP) in mouse primary Müller control cell cultures or after treatment with 100 μM NMDA for 4 h. The actin cytoskeleton was labeled with rhodamine phalloidin (red) and cell nuclei counterstained with 4,6-diamino-2-phenylindole (DAPI) in blue. (C) NMDA-treated Müller cells increase multipotency gene expression in culture. Cells were either left untreated or exposed to 50, 100 or 200 μM for four hours. The graphs show the relative expression values of Nestin, Ascl1 and lin28 calculated based on the 2−ΔΔCt method, from the Ct data obtained by real-time PCR. Means ± SEM of three different experiments performed in three independent biological replicates is shown (unpaired t-test, *p < 0.05).