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. 2023 Jun 21;15:17590914231183272. doi: 10.1177/17590914231183272

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© The Author(s) 2023

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Em micrographs of (A) Müller cell in primary culture, (B-D) extracellular vesicles isolated from control Müller cell cultures supernatants and (E) extracellular vesicles isolated from supernatants from Müller cell cultures that had been treated for 4 h with 100 μm NMDA. (F) Western Blot analysis of extracellular vesicles isolated from control (C-EVs) and NMDA treated cells (N-EVs). 20 μg of protein were loaded per lane. Positive control were EVs obtained from MDA-MB-468 adenocarcinoma line. (G,H) Number of particles and modal size of extracellular vesicles isolated from control or NMDA-treated Müller cells determined by NTA. (-) Negative control: DMEM. (I,J) Graphical representation of the Nanosight analysis of particle size distribution of the EVs isolated from control (I) and NMDA-treated (J) Müller cells. The red shadow is the standard deviation and the black line is the mean of 3 separate analyses.