Table 6.
Studies concerning the production and application of IgY antibodies specific to E. coli.
| Immunogen | Activity/Properties | Reference |
|---|---|---|
| Recombinant FanC protein | In vitro, immunoassays; anti-FanC IgY specifically recognize FanC protein | Nasiri et al. (168) |
| Formaldehyde-fixed EPEC | In vitro, immunoassays; obtained IgYs recognize virulence factors of E. coli | Amaral et al. (169) |
| Recombinant BfpA protein | In vitro, in cellulo; inhibition of E. coli expressing BfpA protein growth in vitro | de Almeida et al. (170) |
| Recombinant BfpA protein | In cellulo; IgY as a tool for describing induction of apoptosis and caspase-3 activation in host cell | Melo et al. (171) |
| OmpA, full recombinant protein and peptide | In vitro, phage display technology, evaluation of the diagnostic potency OmpA scFv antibodies | Mwale et al. (172) |
| Recombinant Tir, EspA, EspB, EspD, and intimin proteins | Porcine ileal in vitro organ culture model; inhibition studies weaned pigs; oral administration, anti-intimin and anti-Tir IgY reduce adherence of E. coli in porcine ileal IVOC, anti-intimin IgY reduce severity of attaching and effacing lesions in the small intestine of pigs |
Girard et al. (173) |
| Recombinant Stx1B protein | Murine model; intraperitoneal injection ensure protective activity against binding Stx1 | Wang et al. (174) |
| Formaldehyde-fixed partially purified recombinant Stx1 and Stx2 | Murine model; intraperitoneal or intravenous injection ensure protective effect of specific IgY, reduced mortality of infected mice | Neri et al. (175) |
| Formaldehyde-fixed Stx2e and recombinant Stx2e B proteins | Murine model; anti-Stx2e IgY and anti-Stx2e B IgY could protect mice from Stx2e challenge; oil adjuvant | Feng et al. (176) |
| O-antigens isolated from LPS od 7 different E. coli strains (O26, O45, O103, O111, O121, O145, and O157) | Synthetic gastric (SGF) and intestinal (SIF) fluids, Caenorhabditis elegans model; chitosan nanoparticles-IgY conjugates act as a specific antimicrobial agent; commercial GeneTel Laboratories adjuvant | Ma et al. (177) |
| Formaldehyde-fixed E. coli O111 | Murine model of endotoxemia; intraperitoneal injection ensure protective effect of specific IgY, reduced mortality of infected mice | Zhen et al. (178) |
| Formaldehyde-fixed ETEC F4 cells, recombinant Bab protein | Murine model; injection; anti-Bab IgY have the capacity to neutralize the toxicity of Sta and STb | You et al. (179) |
| Formaldehyde-fixed K88, K99 and 987P E. coli strains | Weaned pigs; oral administration; hyperimmunized egg yolk powder reduces the E.coli invasiveness; without adjuvant | Han et al. (180) |
| Formaldehyde-fixed K88 (BNCC 125988) | Murine model; oral gavage; prophylactic, ameliorating activity of anti-ETEC IgY, without adjuvant | Han et al. (181) |
| E. coli | Weaned pigs; oral administration; suppression enteric E. coli growth, commercial Zyme Fast (Changsha) Biotechnology adjuvant | Tan et al. (182) |
| E. coli J5 | Newborn calves; oral administration (milk supplementation), after administration of IgY anti-DNT reduction of severity and duration of the diarrhea was observed | Vega et al. (183) |
| Formaldehyde-fixed E. coli K99 | Calves; oral administration (milk supplementation); specific IgY administered with probiotics reduces diarrhea prevalence | Karamzadeh-Dehaghani et al. (184) |
| Killed E.coli, FimH, PapG, IutA, LPS |
Broiler chickens; intramuscular injection; antibodies administered among hens provided protection against homologous challenge; Quil A adjuvant | Kariyanwasam et al. (185) |
| Killed E. coli O78:K80 | Broiler chickens; dietary supplementation; specific IgY improve intestinal condition through immune system | Mahdavi et al. (186) |
| Inactivated whole cells (formaldehyde) and cells fractions | Cattle; average 10 lbs of feed additive per day; infection clearance; no specified data concerning adjuvant | Nash et al. (187) |
| Recombinant FliC and EtpA fusion protein | Murine model; single dose of 50 µl IgY solution 1 h after inoculation; in two of the three groups cells adhesion was inhibited; white oil, aluminum stearate, Span-80 adjuvant | Peng et al. (188) |
| Shiga toxin type 2 recombinant subunit | Murine model; preincubated mixture of toxin and antibody of 0.55 and 8.75 µg/ml; toxin neutralization at higher dose | Parma et al. (189) |
| Inactivated Shiga toxin | Murine model; preincubated mixture of toxin and antibody of 0.5, 1, and 2 mg per mice; dose-dependent toxin neutralization | Fathi et al. (190) |
| Shiga toxin type I recombinant subunit B | Murine model; preincubated mixture of toxin and antibody of 0.4, 1.2, and 3.6 mg; dose-dependent toxin neutralization | Wang et al. (191) |
If not specified in the Activity/Properties column, then FCA/FIA was used as an adjuvant.