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. Author manuscript; available in PMC: 2023 Jun 26.
Published in final edited form as: Brain Behav Immun. 2022 Nov 4;108:80–97. doi: 10.1016/j.bbi.2022.10.024

Figure 3. The MFI immune response is largely complete by 48 hpi.

Figure 3.

Pregnant dams were injected i.p. with 20 mg/kg polyI:C on E11 and E12 to elicit MIA or with saline as a control. Bulk RNA-sequencing was conducted on MFI tissue at E14 (48 hpi). (A) PCA showing clustering of groups from E14 MFI samples. (B) Volcano plots showing the number of differentially expressed genes in E14 MFI tissue comparing polyI:C to saline (FDR<.1), separated by sex. Dark purple dots indicate genes that passed the combined p-value < 1x10−5 and log2FC > 2 cutoff of significance, light purple dots indicate genes with p-value < 1x10−5, and dark grey dots indicate genes with log2FC > 2. (C) qPCR comparing the expression of key MIA-related inflammatory mediator genes between polyI:C and saline MFI tissue at E12 (3 hpi) and E14 (48 hpi). (D) Heatmap representation of the top 20 genes downregulated in the polyI:C group (FDR<.1) compared between saline and polyI:C female MFIs. Statistical significance calculated by unpaired Student’s t-test (C). Error bars indicate mean +/− s.e.m. *P < 0.05, **P < 0.01, ****P < 0.0001.