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. 2023 Jun 26;28:49. doi: 10.1186/s11658-023-00463-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characteristics of tsRNA profiles in LSCC samples (Group A for non-tumor adjacent tissues, Group B for LSCC tissues). A Venn diagrams showed that 281 tsRNAs were co-expressed in both groups. B The Venn diagram shows the overlap number of tsRNAs found in the sequencing data and tRFdb. C Principal component analysis (PCA) showed a clear difference between groups A and B. D Stacked plots indicated the number of subtypes of tsRNAs derived from the same anti-codon tRNA in the two groups. E The bar chart of sequence read length distribution revealed the read counts and the read length for each unique read in LSCC in two groups. F Pie charts show the subtype number of tsRNAs in the two groups