Fig. 5.

The level of lactate and the activity of LDHA were attenuated after tRF^Tyr knockdown. A, B The expression of LDHA in laryngeal cancer cells by western blot analysis. C, D The activity of LDHA (lactate/pyruvic acid) in the indicated cell lines. E, F The level of lactate in the indicated cell lines. G–I The xenograft tumours of the TU212-tRF^Tyr-KD group and TU212-tRF^Tyr-Con group, including the growth curve and tumour volume (n = 6/group). J–K The level of lactate and the activity of LDHA in each indicated xenograft group. L Schematic diagram showing that tRF^Tyr enhances lactate accumulation and promotes the progression of LSCC by binding LDHA