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. 2023 Jun 26;11:142. doi: 10.1186/s40168-023-01582-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Occurrence and phylogeny of C-P lyase clusters in cyanobacterial phycospheres. A The taxonomic constitution of 804 high-quality bins; B C-P lyase gene clusters in ten identified orders of bacteria (groups); C A maximum likelihood phylogenetic tree of phnJ genes from phycospheric heterotrophic bacterial bins and cyanobacteria. A total of 128 phnJ genes from 647 phycospheric heterotrophic bacterial bins and 151 cyanobacterial phnJ genes [25] were used in tree construction. Clades selected for the design of primer set are shown as red (Pseudomonadales), green (Rhodospirillales), and blue (Rhizobiales) branches. Rings from outer to inner are (1) the relative abundance of each phycospheric bacterial bin in each strain sequencing project, (2) bins generated from Microcystis sequencing, and (3) the taxonomic classification of cyanobacterial or heterotrophic bacterial strain for each phnJ gene