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. Author manuscript; available in PMC: 2023 Jun 26.
Published in final edited form as: Dev Cell. 2019 Dec 16;51(6):698–712.e8. doi: 10.1016/j.devcel.2019.11.014

Figure 3. Emergence of Parietal Endoderm (PaE) Subpopulations within PE-like Epithelium.

Figure 3.

(A) Peri-implantation changes in blastocyst morphology. Emergence of primitive endoderm (PE; blue), PaE (light blue), and visceral endoderm (VE; dark blue). Epi, epiblast; TE, trophectoderm; pTE, polar TE; mTE, mural TE.

(B) EPS-blastoid with PDGFRa+ PaE-like cells.

(C) EPS-blastoid built from nuclear PDGFRa-H2B-GFP EPSCs and ubiquitous-eGFP TSCs. Arrowheads show PDGFRa and/or GATA6+ PaE-like cells extending along blastocoel cavity. Left and middle images show stack of 5 Z-planes. Boxed insets (labelled 1 and 2) on the right show Col IV (red) expression between PaE-cell and TSCs in single Z-plane. Orthagonal YZ view shown in inset 2 for better visualization.

(D) Natural E4.75 blastocyst showing early PaE formation (arrowheads).

(E) Percentage of cells expressing PaE-related genes as identified by sc-RNA-seq within the PE-like cell cluster in EPS-blastoids.

(F) The percentage and average expression levels of genes associated with PaE fate commitment within PE-like cell cluster in EPS-blastoids over ES-blastoids.

(G) Representative image of ESC-derived blastocyst in which there is no PaE formation.

Scale bars represent 20 μm in all panels.