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. 2023 Jun 23;11:e15618. doi: 10.7717/peerj.15618

Figure 2. Effects of Lut supplementation during IVM on the developmental competence after parthenogenetic activation (PA).

Figure 2

(A) Representative images of day 6 PA embryos from control and Lut-treated oocytes. Bar = 200 µm. (B) Cleavage rates and (C) blastocyst formation rates of embryos from control and Lut-treated oocytes after PA (Con; n = 208, Lut; n = 221). (D) Representative images of blastocysts and (E) quantification of the proportion of each blastocyst stage from control and Lut-treated oocytes after PA (Con; n = 77, Lut; n = 111). Bar = 200 µm. (F) Representative images of CDX2 staining. Bar = 50 µm. (G) Quantification of the inner cell mass (ICM) and trophectoderm (TE) as well as total cell numbers (n = 30 per group). (H) Representative images of the TUNEL assay results. Green and blue fluorescence indicate TUNEL-labeled embryos (white arrow) and nuclei, respectively. Bar = 50 µm. Quantification of the (I) apoptotic cell number and (J) apoptosis rate (n = 35 per group). Data are from five independent experiments and different superscript letters indicate significant differences (P < 0.05).