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. 2023 Jun 26;222(9):e202207060. doi: 10.1083/jcb.202207060

Figure 7.

Figure 7.

Increasing PCNA levels on DNA in nup170∆ cells rescues subtelomeric silencing defects. (A) Enrichment of differentially expressed genes in the nup170∆ and elg1∆ strains according to gene distance from chromosome ends. X axis: distance from telomere in 25-kb bins. Y axis: negative log10 of the enrichment test’s FDR-adjusted P value. Dashed lines represent significance cutoff. (B) Heatmap showing expression profiles of subtelomeric genes in the nup170∆ and elg1∆ strains. (C) Gene expression levels of four subtelomeric genes were measured in the WT, elg1∆, nup170∆, and nup170 elg1∆ strains by RT-qPCR, and RQ values are plotted. The RQmin/RQmax values are presented as error bars. Data from at least three biological replicates are presented. (D) PCNA enrichment at the indicated ARS sites was analyzed by qPCR. Means ± SD from three experiments are shown; *P < 0.05, **P < 0.01.