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. 2023 Jun 26;222(9):e202207060. doi: 10.1083/jcb.202207060

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© 2023 Choudhry et al.

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Figure S3. — Ctf18 interaction with the NPC peaks during S phase. (A and B) Cells producing Ctf18-GFP and Nup170-3FLAG were arrested in G1 and samples were collected at indicated time points after release. The Ctf18-GFP fusion protein was affinity-purified from cell lysates and the eluate was analyzed by immunoblotting to detect Nup170-3FLAG. The cell cycle stages are indicated based on G1 arrest and Clb2 (indicative of G2 stage) levels in cell lysates. Anti-FLAG, Clb2, and G6PDH antibodies were used for immunoblotting. A and B are biological replicates, and B is the technical replicate of the Fig. 4 D. MW, molecular weight. Source data are available for this figure: SourceData FS3.