FIGURE 3.

Impact of reduced dietary Mg²⁺ on plasma and urine Mg²⁺ in LM/Bc and SWV mice. The impact of reduced dietary intake of Mg²⁺ on plasma Mg²⁺ levels (median mmol/L ± 95% CI) in LM/Bc mice are shown in (A). Plasma samples were evaluated in weaned females on high Mg²⁺ (0.3%) soy diet (blue dots) and 15 days after they were placed on the high Mg²⁺ (0.3%) casein diet (red dots). There was no significant change in plasma Mg²⁺ between the 2 high Mg²⁺ (soy and casein) diets. However, after LM/Bc mice were placed on the control Mg²⁺ (0.05%) casein diet for 15 days or 60 days (green dots), plasma Mg²⁺ dropped significantly when compared to either of the high Mg²⁺ diets. For all plots in Figure 3 (*p < .0.05, **p < 0.01, ***p < 0.01, ****p < 0.0001). The impact of reduced dietary intake on urinary excretion of Mg²⁺ (µmol/24 h ± 95% CI) for the corresponding diets and time points in LM/Bc mice is shown in (B). After 15 days on the high Mg²⁺ casein diet (red dots), urinary Mg²⁺ excretion was significantly higher than on either the high Mg²⁺ soy diet (blue dots) or the control Mg²⁺ casein diet (green dots). After 15 days or 60 days on the control Mg²⁺ (0.05%) casein diet, urinary Mg²⁺ output was similar and not significantly different from the high Mg²⁺ soy diet. The impact of severely reduced dietary intake of Mg²⁺ on plasma Mg²⁺ levels (median mmol/L ± 95% CI) in SWV mice is shown in (C). Plasma samples were evaluated in weaned females on high Mg²⁺ (0.3%) soy diet (blue dots) and again 15 days after they were placed on the high Mg²⁺ (0.3%) casein diet (red dots); somewhat unexpectedly, plasma Mg²⁺ levels decreased on the high Mg²⁺ (0.3%) casein diet (p < .05). After 15 days on Mg²⁺ deficient (<0.003%) diet, plasma Mg²⁺ levels dropped significantly (purple dots) relative to either of the high Mg²⁺ diets. The impact of reduced dietary intake on urinary excretion of Mg²⁺ (µmol/24 h ± 95% CI) for the corresponding diets and time points in SWV mice is shown in (D). Like the trend observed in LM/Bc mice, urinary Mg²⁺ excretion in SWV mice increased significantly after 15 days on the high Mg²⁺.