FIGURE 5.

DTG-NTD dose-response in LM/Bc mice on casein control Mg²⁺ diet. LM/Bc mice were placed on the control Mg²⁺ (0.05%) casein diet for a minimum of 4 weeks prior to timed matings. Pregnant mice on diet only control (no treatment) were compared to mice treated with either DTG500 or DTG750 (oral gavage from GD 0.5—GD 9.5) and litters examined for NTDs. [No NTDs were observed in litters from an additional n = 6 dam on control Mg²⁺ diet treated with vehicle (data not shown)]. Comparisons shown are between litter means (average % of embryos in each litter that had an NTD). No NTDs were observed in diet only control litters (blue dots). The % DTG-NTD-affected litters (62% for DTG500 and 85% for DTG750) and mean % affected embryos/litter (shown on graph) were both significantly higher than the diet only control group, but DTG500 (red dots) and DTG750 litter means (green dots) were not significantly different from each other. (*p < 0.05, ****p < 0.0001).