TABLE 2.

Strain comparison: NTDs ON HIGH Mg2+ SOY DIET x DTG750. Pregnant dams from three different mouse strains with inherently different basal plasma Mg2+ levels were maintained on a high Mg2+ (0.3%) soy diet and treated with oral vehicle or DTG (750 mg/kg/d) from the day of conception (GD 0.5) until GD 9.5. The strains were compared for susceptibility to DTG-NTDs. The dam is considered the experimental unit and comparisons are between litter means. The mean litter rate was calculated by averaging the % of embryos in each litter that had an NTD. A litter is considered affected if at least 1 embryo presented with exencephaly (NTD). Statistical analysis was done by 2-way ANOVA and post hoc analysis (Fisher least significant difference method) or by the Kruskal–Wallis test for nonparametric analyses of index values, both using the software Sigma XL (https://www.sigmaxl.com/). Within each variable, the highlighted numbers and asterisks indicate statistical differences in pairwise comparisons (α > 0.99).

	LMBc		SWV		C57BL/6J
Mean (SD)	VEH	DTG750	VEH	DTG750	VEH	DTG750
Implants per litter	8.40 (1.65)	8.74 (2.47)	14.17 (1.03)**	13.15 (2.12)**	8.82 (1.47)	9.80 (1.48)
Resorptions per litter	0.90 (0.88)	0.95 (1.27)	1.25 (1.29)	0.62 (0.87)	0.82 (1.08)	0.50 (0.85)
Total viable embryos	7.50 (1.08)	7.79 (2.76)	12.92 (1.08)**	12.54 (2.37)**	8.00 (2.19)	9.30 (1.77)
Dev. delayed	0.00 (0.00)	0.58 (0.96)	0.25 (0.45)	0.31 (0.48)	0.09 (0.30)	0.20 (0.42)
NTDs	0.00 (0.00)	1.47 (1.74)**	0.00 (0.00)	0.23 (0.83)	0.00 (0.00)	0.00 (0.00)
Index Values Average % (SD)
Resorptions/implants	9.6% (8.87)	11.5% (15.75)	8.5% (8.50)	5.0% (7.62)	10.8% (14.81)	5.3% (9.30)
Dev delay/implants	0.0% (0.00)	8.1% (17.76)	1.7% (3.16)	2.0% (3.19)	1.0% (3.35)	1.9% (4.15)
NTDs/implants	0.0% (0.00)	17.7% (20.63)**	0.0% (0.00)	1.6% (5.94)	0.0% (0.00)	0.0% (0.00)
NTDs/embryos	0.0% (0.00)	20.9% (23.65)**	0.0% (0.00)	1.8% (6.40)	0.0% (0.00)	0.0% (0.00)
Affected litters %	0.0%	63.2%**	0.0%	7.7%	0.0%	0.0%
Number of litters	10	19	12	13	11	10