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Primer Sequences for qPCR
| Gene | Primer Sequence (5′–3′) |
|---|---|
| GSDMD | Forward: TCATGTGTCAACCTGTCAATCAAGGACAT |
| Reverse: CATCGACGACATCAGAGACTTTGAAGGA | |
| P2X7 | Forward: CCAAGCCGACGTTGAAGTAT |
| Reverse: AGAGCTTGGAGGTGGTGATG | |
| Caspase-1 | Forward: GTACACGTCTTGCCCTCATTATCTG |
| Reverse: TTTCACCTCTTTCACCATCTCCAG | |
| NLRP3 | Forward: CTTCTAGCTTCTGCCGTGGTCTCT |
| Reverse: CGAAGCAGCATTGATGGGACA | |
| GAPDH | Forward: CATGGCCTCCAAGGAGTAAGA |
| Reverse: GAGGGAGATGCTCAGTGTTGG |
