Table 6.
Carbapenemases identified in carbapenem-resistant Gram-negative bacteria isolated on selective culture by NG Carba5 rapid test and genes encoding for carbapenemases identified in carbapenem-resistant Gram-negative bacteria isolated on selective culture by home-made PCR.
| Variables | Carbapenem-resistant K.pneumoniae (N 67) |
Carbapenem-resistant E.coli (N 37) |
Carbapenem-resistant A.baumanni (N 21) |
Total number of carbapenem-resistant strains (N 125) |
|---|---|---|---|---|
| Positivity by NG Carba5 rapid test | ||||
| Number of strains positive for at least one carbapenemase, n (%) | 59 (88%) | 19 (51%) | 0 (0%) | 78 (62%) |
| Type of expressed carbapenemase KPC, n (%) |
48 (81%) | 9 (47.5%) | 0 | 57 (73%) |
| VIM, n (%) | 9 (15%) | 9 (47.5%) | 0 | 18 (23%) |
| NDM, n (%) | 1 (2%) | 1 (5%) | 0 | 2 (2.5%) |
| OXA-48like, n (%) | 1 (2%) | 0 | 0 | 1 (1.5%) |
| OXA-23like, n (%) | 0 | 0 | 0 | 0 |
| OXA-24like, n (%) | 0 | 0 | 0 | 0 |
| Total number of expressed carbapenemases | 59 (100%) | 19 (100%) | 0 | 78 (100%) |
| Positivity by home-made PCR | ||||
| Number of strains positive for at least one gene encoding for carbapenemase, n (%) |
62 (92.5%) | 36 (97%) | 20 (95%) | 118 (94.4%) |
| blaKPC, n (%) | 51*# (82%) | 24‡ ± (66%) | 0 | 75 (64%) |
| blaVIM, n (%) | 11#† (18%) | 14‡ (39%) | 0 | 25 (21%) |
| blaNDM, n (%) | 4*† (6%) | 2± (6%) | 0 | 6 (5%) |
| bla-OXA48like, n (%) | 1† (2%) | 0 | 0 | 1 (1%) |
| bla-OXA23like, n (%) | 0 | 0 | 18° (90%) | 18 (15%) |
| bla-OXA24like, n (%) | 0 | 0 | 20° (100%) | 20 (17%) |
| Total number of identified genes encoding for carbapenemases• | 67 (108%)• | 40 (111%)• | 38 (190%)• | 145 (123%)• |
*Two strains harbouring at the same time blaKPC and blaNDM; #one strain harbouring at the same time blaKPC and blaVIM; † one strain harbouring at the same time blaOXA-48like, blaVIM and blaNDM; ‡ 3 strains harbouring at the same time blaKPC and blaVIM; ± 1 strain harbouring at the same time blaKPC and blaNDM; ° 18 strains harbouring at the same time blaOXA-23 and blaOXA-24; • more than 100% because some strains harbouring at the same time more than one gene for carbapenemase.