Mutation |
Primer name |
Sequence (5’ - 3’) |
V410L |
Val410fw |
GCG GGC AGG GCG GCG GGG GCG GGG CCA TCT TCT TGG GTT CGT TCT ACC GTG |
Leu410fw |
GCG GGC ATC TTC TTG GGT TCG TTC TAC CAT T |
410rev |
TTC TTC CTC GGC GGC CTC TT |
F1534C |
Cys1534fw |
GCG GGC AGG GCG GCG GGG GCG GGG CCT CTA CTT TGT GTT CTT CAT CAT GTG |
Phe1534fw |
GCG GGC TCT ACT TTG TGT TCT TCA TCA TAT T |
1534rev |
TCT GCT CGT TGA AGT TGT CGA T |
V1016I |
Val1016fw |
GCG GGC AGG GCG GCG GGG GCG GGG CCA CAA ATT GTT TCC CAC CCG CAC CGG |
Ile1016fw |
GCG GGC ACA AAT TGT TTC CCA CCC GCA CTG A |
1016rev |
TGA TGA ACC SGA ATT GGA CAA AAG C |