Figure 9.

Combined chemo-radiotherapy with the ¹⁷⁷Lu-PLGA(RGF)-CXCR4L nanosystem inhibits cell survival and proliferation of HCT116 cancer cells through a decrease of Akt and Erk activation. (A) The colorectal cancer cell line HCT116 was treated with ¹⁷⁷Lu-PLGA(RGF)-CXCR4L and its respective controls for 24 h (RGF at a concentration of 7.26 μM and 20 μCi of ¹⁷⁷Lu). (B) Cells were treated with ¹⁷⁷Lu-PLGA(RGF)-CXCR4L and its respective controls in a clonogenic assay; blue-violet colonies are viable cells. Representative images of the clonogenic assay of three independent experiments are shown. Quantification of crystal violet staining from HCT116 cancer cells depicted in (C) n = 3. ^***p < 0.0001. (D) WB-analyzed cell lysates for phosphorylation levels of pErk1/2, pAkt, and GAPDH as a loading control. Representative blots of three independent assays are shown.