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. 2023 Jun 12;15(11):4667–4684. doi: 10.18632/aging.204793

Figure 2.

Figure 2

Expression patterns of selected genes during differentiation of C2C12 myotubes. (A) Relative gene expression of target genes in differentiating C2C12 cells at 0-, 1-, 2-, 3-, and 5-days post-induction. mRNA levels were normalized to 36b4 mRNA levels, as measured by qPCR analysis. The data are represented as mean ± SEM. Statistically significant differences are denoted as *p < 0.05, **p < 0.01, ***p < 0.005 vs. DM0. (B) Representative images of myosin heavy chain (MHC; green) immunostaining in myoblasts transfected with target genes and differentiated for 5 days. (C) Quantification of myotube myosin-positive (Myh+) areas, (D) myotube diameter, and (E) fusion index. (F) Western blot showing expression of MHC and myogenin, differentiation markers, during differentiation (0-, 1-, 2-, and 3-days). The data are represented as mean ± SEM. Statistically significant differences are denoted as *p < 0.05, **p < 0.01, ***p < 0.005 vs. si-Con.