Figure 4.

Selected genes regulate mitochondrial respiration. (A) The real-time oxygen consumption rate (OCR) of selected gene-transfected C2C12 myotubes was determined using a Seahorse XFe96 analyzer. (B) The area under the curve (AUC) values of basal respiration, ATP-linked respiration, and maximal mitochondrial respiration. OCRs were normalized to total cellular protein. All data are represented as mean ± SEM. Statistically significant differences are denoted as ^*p < 0.05, ^**p < 0.01, ^***p < 0.005.