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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: Fertil Steril. 2023 Mar 27;120(1):163–174. doi: 10.1016/j.fertnstert.2023.02.034

Figure 3.

Figure 3.

(A) Distribution of mean methylation across DMRs. (B) THC-exposed rhesus vs. human cannabis users. Rhesus pre-THC vs. heavy-THC DMRs which overlap genes nearest DMCs in human cannabis users vs control (Left). Post-THC DMR genes in rhesus macaques overlap with Post-cannabis DMC genes in humans (Right). (C) Identification of DMRs pre-THC versus heavy-THC restored with THC washout (Left) and persistent DMRs (Right). Heatmaps represent the log transformed and mean centered average methylation values per DMR which intersect DMRs between pre-THC and heavy-THC. Hierarchical clustering demonstrates overall similarity in means between groups. (D) Significant overlap of genes annotated to THC DMRs with candidate autism genes. Venn diagrams showing the number of genes that are differentially methylated in sperm after heavy-THC versus pre-THC which overlap (Left) genes included on the SFARI autism candidate list with homologs in rhesus macaques, and (Right) genes with DMRs in sperm from fathers of children with autism versus without (27).