Fig. 2.

Multivariate analysis of metabolites for zebrafish crush and control optic nerve groups 3 days post crush. (A) Principal component analysis (PCA) 2-D scores plot of metabolite profiling data. (B) Partial Least Squared Discriminant Analysis with associated Variable Importance in Projection (VIP) scores. The colored boxes on the right indicate the relative concentrations of the corresponding metabolite in each group. Red indicates high concentration and blue indicates low concentrations. (C) Volcano plot of significant up and down regulated metabolites (FDR<0.05, FC>2). Labels are provided per metabolites. Red labeling indicates increased Crush/Control fold change while blue indicates decreased Crush/Control fold change. gray dots are non-significant metabolites.