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. 2023 May 11;48:109228. doi: 10.1016/j.dib.2023.109228

WGS of Streptomyces californicus TBG-201: Specifications table

Subject Biological science
Specific subject area Microbiology, Bacterial Genomics, Microbial biotechnology.
Type of data Whole genome sequence data, predicted genes, and functional analysis of respective proteins, figure, table.
How the data were acquired De novo sequencing was performed using the Illumina HiSeq-2500 sequencing platform. Genome assembled using ABySS v. 2.0.1, MaSuRCA v. 2.3.2, and Velvet v. 1.2.10. Genome annotation was done by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP).
Data format Raw and analyzed.
Description of data collection The modified CTAB method was used to extract the genomic DNA of S. californicus TBG-201, and a genomic library was prepared using the Illumina TruSeq Nano DNA Library Prep Kit. The genome was sequenced on the Illumina HiSeq-2500 platform, utilizing a 2 × 150bp pair-end protocol. Genome assembly was carried out using ABySS version 2.0.1, MaSuRCA version 2.3.2, and Velvet version 1.2.10. The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) was employed to perform genome annotation, utilizing the best-placed reference protein set. Prediction of biosynthetic gene clusters was conducted using AntiSMASH, while identification of carbohydrate-active enzymes was performed using CAZy analysis via the dbCAN meta server.
Data source location
  • Institution: Department of Botany, Kariyavattom Campus, University of Kerala, Trivandrum, Kerala, India.

  • City/Town/Region: Vandanam sacred groves, Alleppey District, Kerala.

  • Country: India.

  • Latitude and longitude for collected samples/data: 9.4946oN, 76.3311oE. Altitude- 6 m.

Data accessibility Repository name: NCBI GenBank
Bioproject: PRJNA772892
NCBI BioSample: SAMN22418706
NCBI GenBank Accession Number: JAJDST000000000
Assembly: ASM2064085v1
Direct URL to data: https://www.ncbi.nlm.nih.gov/nuccore/JAJDST000000000
All additional data and supplementary files may be accessed at Mendeley
data: https://data.mendeley.com/datasets/fgtz42yfh7,
DOI:10.17632/fgtz42yfh7.1