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. 2023 Jun 13;14:1192035. doi: 10.3389/fmicb.2023.1192035

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Copyright © Punja, Ni, Lung and Buirs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of three agar media for the recovery of yeasts and molds originating from fresh and dried cannabis samples. The three media tested were PDA (potato dextrose agar containing 140 mg/L streptomycin sulfate), SDA (Sabouraud dextrose agar) and TSA (tryptic soy agar). The same sample source was used for the fresh and dried tissue analysis. (A,B) The results from six cannabis samples are shown, where each sample is represented by a different plant source and/or genotype. The samples were run in duplicate and the extracts were plated onto 4 Petri dishes (n = 8) for each sample. The number of colonies per Petri dish was rated after 5 days of incubation at 23°C. The vertical bars represent means for each sample with standard error bars reflecting 95% confidence intervals around the mean. Dried tissues showed consistently lower colony numbers compared to fresh samples. (C–F) The morphological appearance of colonies of yeasts and molds on the three different media tested. Results from samples (A,B,E) are shown. In each row, the top dish contains PDA + S, the middle dish contains SDA and the bottom dish contains TSA. While colony numbers were similar across all media, morphological differences between colonies of different species were most clearly seen on PDA + S. Colonies of TSA were much smaller in size compared to the other two media. In the far right panel, Fusarium was plated out to show the more distinct colony color and morphology on PDA + S compared to the other two media.