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. Author manuscript; available in PMC: 2023 Jun 27.
Published in final edited form as: Neurobiol Dis. 2021 Jan 9;150:105258. doi: 10.1016/j.nbd.2021.105258

Fig. 8. Effects of GFAP/caspase-8 on induction of astroglial necroptosis.

Fig. 8.

A. Immunolabeling of retinal whole mounts demonstrated phosho-RIPK3 and phospho-MLKL expression (red) of GFAP-labeled astroglia (green) in ocular hypertensive eyes of GFAP/caspase-8 mice compared to caspase-8f/f ocular hypertensive controls. B. Immunoblots of isolated astroglia proteins with antibodies to phosho-RIPK3 or phospho-MLKL also supported up-regulated expression of these necroptosis markers in GFAP/caspase-8 mice with experimental glaucoma (G) compared to caspase-8f/f ocular hypertensive controls (C). Data (mean ± SD) were obtained using a minimum of three new samples per group. P values (obtained using a one-way ANOVA) for the statistical comparison of experimental groups are given in each graph. C. Similar to retinal whole mounts, immunolabeling of the histological sections of optic nerve head tissue in these animals was also supportive of the induction of astroglial necroptosis in GFAP/caspase-8 mice. D. However, immunolabeling of retinal whole mounts did not detect phospho-MLKL expression in rat eyes injected with z-IETD-fmk or vehicle (scale bar, 100 μm).