Fig. 5.

AICAR can reverse the inhibition of AMPK phosphorylation due to Enpp1 deficiency: A Western blot of chondrocytes (WT, WT + AICAR, Enpp1^−/−, Enpp1^−/− + AICAR, respectively; p < 0.05, n ≥ 3)); B–D quantitative statistic of Western blot (p < 0.05, n ≥ 3); E TUNEL staining of chondrocytes (WT, WT + AICAR, Enpp1^−/− and Enpp1^−/− + AICAR, respectively; p < 0.05, n ≥ 3); F Quantitative analysis of TUNEL-positive chondrocytes (p < 0.05, n ≥ 3). AICAR, Acadesine; AMPK, AMP-activated protein kinase; Enpp1, ectonucleotide pyrophosphatase/phosphodiesterase 1; WT, wild type; TUNEL, TdT-mediated dUTP-biotin nick end labeling; DAPI, 4′,6-diamidino-2-phenylindole