Figure 2.

Representative micrographs demonstrating rarefaction and vacuolization in the postmortem brain.

Tissue from the frontal cortex tissue of an 87-year-old man with a PMI of 28 hours. A: H&E/LFB (Luxol fast blue)-stained image demonstrating a perivascular rarefaction in the grey matter. Arrowheads denote examples of intertwining fibers. Scale bar = 10 μm. B: H&E/LFB-stained image showing variable degrees of asymmetric pericellular rarefactions (asterisks). Scale bar = 20 μm. C: H&E/LFB-stained image of white matter vacuoles (asterisks) and pericellular rarefactions. White matter vacuoles are found haphazard in distribution, but appear more common near blood vessels, likely because of postmortem fluid extravasation. Scale bar = 20 μm. D: Electron photomicrograph in grey matter demonstrates prominent electron lucent areas that appear to be swollen cellular processes (asterisks). Scale bar = 500 nm.