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. Author manuscript; available in PMC: 2023 Jun 27.
Published in final edited form as: Nat Neurosci. 2022 Aug 18;25(9):1179–1190. doi: 10.1038/s41593-022-01135-0

Extended Data Fig. 4. Mutations in tgMOR; bgg10 occur in PTR-25/F43D9.1, a conserved member of the Patched protein family.

Extended Data Fig. 4.

a, Plot showing analysis of whole genome sequence data with mapped region of chromosome III (red and grey bars) containing bgg10. b, Gene diagram showing Q1032stop nonsense mutation (red arrow) in ptr-25 (F43D9.1) contained in mapped region from tgMOR; bgg10 mutants. c, PTR-25 protein with Sterol Sensing Domain (SSD, yellow), Patched family domain (blue) and arginine rich domain (grey). bgg10 mutation, Q1032stop, highlighted (red arrow). d, Phylogenetic analysis of Patched family proteins including all major subfamilies: PTC/PTCH, NCR/NPC, DISP/PTCHD2, PTCHD1/4 (shaded in green) and the PTR group which is heavily expanded in C. elegans. Note PTR-25 has closest homology to mammalian PTCHD1/4. Protein sequences from human (black), mouse (light blue), fly (dark blue) and C. elegans (red) were aligned with Clustal W. Phylogenetic tree was constructed using maximum likelihood method with 1000 bootstrap replicates.