TABLE 2.
Abridged results of IP-MS analysisa
| Expt | Strain | Protein | Peptide count |
|
|---|---|---|---|---|
| −IAA | +IAA | |||
| Pulldown HIP | HIP-mAID-HA (HOOK-Ty) | HOOK | 12 | NA |
| HIP-mAID-HA (HOOK-Ty) | FTS | 0 | NA | |
| HIP-mAID-HA (HOOK-Ty) | HIP | 5 | NA | |
| Pulldown HOOK | HOOK-Ty (HIP-mAID-HA) | HOOK | 45 | 41 |
| HOOK-Ty (HIP-mAID-HA) | FTS | 7 | 7 | |
| HOOK-Ty (HIP-mAID-HA) | HIP | 4 | 0 | |
| Pulldown FTS | FTS-mAID-HA | HOOK | 39 | NA |
| FTS-mAID-HA | FTS | 9 | NA | |
| FTS-mAID-HA | HIP | 8 | NA | |
a
Mass spectrometry was performed on coimmunoprecipitated material (±IAA 24 h) and the total number of identified peptides was quantified. NA, Non applicable.