TABLE 5.
Primers used in this study
| Namea | Sequence | Purpose |
|---|---|---|
| Pho4-yEGFP_f | CTGCCGGTACATCCGTCACCTACAGCAGAACGTGAGCACGggtgacggtgctggttta | Pho4-yEGFP tagging |
| Pho4-yEGFP_r | AGTCCGATATGCCCGGAACGTGCTTCCCATTGGTGCACGGtcgatgaattcgagctcg | |
| Pho4-yEGFP_colony_f | CCGCTCGCACGGAAATATTT | |
| Pho4-yEGFP_colony_r | ACTAAGGTATCACCTTCAAAC | |
| Vtc4_guide_f | CGAAGATAACGACTTCGATGGTTTTAGAG | Vtc4 deletion |
| Vtc4_guide_r | CTAGCTCTAAAACCATCGAAGTCGTTATCTTCGACGT | |
| Vtc4_HR_f | AAATCGGCCAATAAAAGAGCATAACAAGGCAGGAACAGCTATACACAGCGTGTTTTTTTTACTGTATAATTAAGTAATAA | |
| Vtc4_HR_r | TTATTACTTAATTATACAGTAAAAAAAACACGCTGTGTATAGCTGTTCCTGCCTTGTTATGCTCTTTTATTGGCCGATTT | |
| Vtc4_colony_f | GACGGAGAGCTACTGACTTGT | |
| Vtc4_colony_r | TGTGATGGTGACGATGGCATG | |
| Taf10_qRT_f | ATATTCCAGGATCAGGTCTTCCGTAGC | qRT-PCR |
| Taf10_qRT_r | GTAGTCTTCTCATTCTGTTGATGTTGTTGTTG | |
| Pho5_qRT_f | GGGCAACACTTTCCACAGAT | |
| Pho5_qRT_r | CAATTGGAACAACAGCATCG | |
| Pho84_qRT_f | TTCTGCTGCATCTGGTAAGG | |
| Pho84_qRT_r | TCCATGACGTGAGGTAACCA |
a
f, forward; r, reverse; HR: homologous recombination.