FIG 5.

Decreasing NA activity increases local virion shedding. (A) Quantification of local virion shedding under continuous treatment with exogenous sialidase (CpNA). The data are combined from three biological replicates (at least 10 sites of infection per replicate). The P values are determined from independent t tests. (B) Quantification of cis and trans cleavage of Sia by WSN33 and WSN^LS. The data are combined from three biological replicates (at least five sites of infection/replicate). The P values are determined from independent t tests. (C) Quantification of local virion shedding for WSN33 and WSN^LS. The data are combined from three biological replicates (at least 11 sites of infection/replicate). The P values are determined from independent t tests. (D) Comparison of virions detected in the supernatant (average per HA-positive cell) between WSN33 and WSN^LS. The absolute value of WSN33 is not comparable to results shown previously as they were performed on different days. The data are combined from three biological replicates. The error bars represent standard deviation. The P values are determined by independent t tests. (E) Quantification of local virion shedding for viruses with WSN33 NA (in WSN33 or CA09 backgrounds) under oseltamivir treatment. The data are combined from three biological replicates (at least 10 sites of infection/replicate). The P values are determined from independent t tests. (F) Quantification of local virion shedding for viruses with CA09 NA (in WSN33 or CA09 backgrounds) under oseltamivir treatment. The data are combined from three biological replicates (at least 10 sites of infection/replicate). The P values are determined from independent t tests.