FIG 2.

Disruption of tli increases cell permeability. (A) ECL is impermeable to chromogenic X-Gluc substrate. Wild-type and cdi-gusA reporter cells were grown on LB agar supplemented with X-Gluc. Where indicated, SDS solution (1% [wt/vol]) was applied to the edge of the colonies prior to imaging. (B) The indicated ECL strains were complemented with plasmid-borne tli (pTli) or an empty vector and grown on LB agar supplemented with X-Gluc. (C) Complementation with tli does not affect cdi-gusA reporter expression. β-Glucuronidase activities were quantified as Miller units as described in Materials and Methods. Data for technical replicates from two independent experiments are shown with the average ± standard deviation.