FIG 3.

Hyperpermeability is T6SS-1 dependent. (A) Schematic of the ECL T6SS-1 locus. Core secretion system genes are colored light orange, and effector and immunity genes are shown in red and green, respectively. Genes of unknown function are not colored and are indicated by their ordered locus number. The extents of the gene deletions used in this study are indicated. (B) ECL ΔtssM strains do not secrete Hcp. The indicated ECL strains were grown in LB, and then cell pellet (P) and culture supernatant (S) fractions were prepared as described in Materials and Methods. Where indicated, the strains carried a plasmid-borne copy of tssM (pTssM) or an empty vector plasmid. Fractions were analyzed by SDS-PAGE and immunoblotting with polyclonal antisera to Hcp. (C) ECL tli ΔtssM mutants do not exhibit increased cell permeability. The indicated ECL strains were grown on LB agar supplemented with X-Gluc. Where indicated, the strains carried pTssM or an empty vector plasmid.