TABLE 1.
Bacterial strains used in this study
| Strain | Descriptiona | Reference or source |
|---|---|---|
| CH1360 | ECL Δtle-tli-vgrG1-vgrG2::kan Kanr | This study |
| CH2016 | E. coli X90(DE3) Δrna ΔslyD::kan Kanr | 73 |
| CH3469 | ECL Δtli glmS::cdi-gusA Gentr | This study |
| CH11196 | ECL ΔtssM1::kan Kanr | 27 |
| CH11199 | ECL Δhcp1::spc Specr | 29 |
| CH11248 | ECL araC-PBAD::cdiB ΔaraBAD::kan Specr Kanr | 27 |
| CH11396 | ECL ΔtssM1 | 29 |
| CH11876 | ECL Δtle::kan Kanr | 29 |
| CH11895 | ECL Δtle Δtli::spc Specr | 29 |
| CH12384 | ECL ΔvgrG1::kan Kanr | 28 |
| CH14384 | ECL Δtle Δtli::spc rif Specr Rifr | This study |
| CH14587 | ECL Δtli::kan Kanr | This study |
| CH14588 | ECL Δtli | This study |
| E2072 | E. coli F– λ– mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 galU galK rpsL nupG ΔdapA::pir-lacIq-Gentr-FRT8 mob[recA::RP4-2 Tc::Mu-Kanr] leu+ Strr Gentr Kanr | 75 |
| ECL | Enterobacter cloacae subsp. cloacae ATCC 13047 | ATCC |
| MFD pir | E. coli MG1655 RP4-2-Tc::[ΔMu1::aac(3)IV-ΔaphA-Δnic35-ΔMu2::zeo] dapA::(erm-pir) ΔrecA Aprr Zeor Ermr | 76 |
| X90 | E. coli F′ lacIq lac′ pro′/ara Δ(lac-pro) nal1 argE(Amb) rif thi-1 Rifr | 77 |
| ZR71 | ECL glmS::cdi-gusA Gentr | This study |
| ZR135 | ZR71 tli-231; Tn5 insertion after tli nucleotide 231; Gentr Specr | This study |
| ZR137 | ZR71 tli-21; Tn5 insertion after tli nucleotide 21; Gentr Specr | This study |
| ZR138 | ZR71 tli-57; Tn5 insertion after tli nucleotide 57; Gentr Specr | This study |
| ZR139 | ZR71 tli-663; Tn5 insertion after tli nucleotide 663; Gentr Specr | This study |
| ZR140 | ZR71 tli-17; Tn5 insertion after tli nucleotide 17; Gentr Specr | This study |
| ZR148 | ZR71 tli-231 ΔtssM::kan Gentr Specr Kanr | This study |
| ZR149 | ZR71 tli-21 ΔtssM::kan Gentr Specr Kanr | This study |
| ZR150 | ZR71 tli-57 ΔtssM::kan Gentr Specr Kanr | This study |
| ZR151 | ZR71 tli-663 ΔtssM::kan Gentr Specr Kanr | This study |
| ZR152 | ZR71 tli-17 ΔtssM::kan Gentr Specr Kanr | This study |
| ZR161 | ZR71 tli-31; Tn5 insertion after tli nucleotide 31; Specr Gentr | This study |
| ZR163 | ZR71 tle-1106; Tn5 insertion after tle nucleotide 1106; Specr Gentr | This study |
| ZR165 | ZR71 tli-673; Tn5 insertion after tli nucleotide 673; Specr Gentr | This study |
| ZR170 | ZR71 tli-64; Tn5 insertion after tli nucleotide 64; Specr Gentr | This study |
| ZR200 | ZR71 ΔtssM::kan Gentr Kanr | This study |
a
Aprr, apramycin resistant; Ermr, erythromycin resistant; Kanr, kanamycin resistant; Gentr, gentamicin resistant; Rifr, rifampin resistant; Specr, spectinomycin resistant; Strr, streptomycin resistant; Zeor, zeocin resistant.