TABLE 2.
Plasmids used in this study
| Plasmid | Descriptiona | Reference |
|---|---|---|
| pBR322 | Cloning vector; Ampr Tetr | 78 |
| pCH70 | pBluescript with FRT-flanked Kanr cassette ligated into SmaI restriction site; Ampr Kanr | 79 |
| pCH371 | pCATKAN-pheS(A294G); Cmr Kanr | This study |
| pCH377 | pCAT-pheS(A294G); Cmr | This study |
| pCH417 | pCH450::vgrG2(ECL)-CT/vgrI(Eh49162); Tetr | This study |
| pCH450 | pACYC184 derivative with E. coli araC and araBAD promoter for arabinose-inducible expression; Tetr | 68 |
| pCH494 | pCH450K::tli; Tetr | 29 |
| pCH495 | pCH450K::tli(Δ23); Tetr | This study |
| pCH1359 | pCATKAN-pheS(A294G)-Δtle-tli-vgrG1-vgrG2; Cmr Kanr | This study |
| pCH3128 | pCH450::tle-tli; Tetr | This study |
| pCH3291 | pET21::tli(Δ23); Ampr | This study |
| pCH3468 | pUC18T-mini-Tn7T-Gm with the ECL cdi promoter region fused to E. coli gusA within mini-Tn7; Ampr Gentr | This study |
| pCH3763 | pCH450::vgrG2-lipase(S341A)-tli; Tetr | This study |
| pCH3884 | pCATKAN-pheS(A294G)-Δtli; Cmr Kanr | This study |
| pCH5036 | pCH450K::tli(Δ51); Tetr | This study |
| pCH5037 | pCH450K::tli(Δ75); Tetr | This study |
| pCH6118 | pET21b::cdiB(STEC3); Ampr | This study |
| pCH9062 | pCH450::vgrG2-lipase-tli; Tetr | This study |
| pCH9063 | pCH450::vgrG2-lipase; Tetr | This study |
| pCH9384 | pBluescript with FRT-flanked Specr cassette ligated into SmaI restriction site; Ampr Specr | 38 |
| pCH11050 | pCH70 with regions upstream and downstream of ECL tssM1; Ampr Kanr | 27 |
| pCH11198 | pCH450::tssM; Tetr | This study |
| pCH14212 | pCH450::tle; Tetr | This study |
| pCH15269 | pSH21P::lipase; Ampr | This study |
| pCP20 | Temp-sensitive replication origin, expresses FLP recombinase; Ampr Cmr | 80 |
| pDAL912 | pBR322::pheS(A294G); Ampr | This study |
| pDAL6480 | pRE118-pheS(A294G); Kanr | This study |
| pKOBEG | Temp-sensitive replication origin, expresses the Bet-Gam-Exo proteins from phage λ; Cmr | 69 |
| pLG99 | Tn5 transposase expression vector harboring a mini-Tn5 derivative with PrhaB-out; Ampr Tmpr | 70 |
| pLG99-spec | pLG99 with Specr cassette cloned into Tn5; Ampr Specr | This study |
| pRE118 | Vector plasmid for sacB allelic exchange; Kanr | 67 |
| pSCBAD | pBBR1 derivative that carries araC and PBAD promoter; Tmpr | 72 |
| pTNS2 | Expresses Tn7 transposase, Ampr | 66 |
| pUC18T-mini-Tn7T-Gm | Tn7 transposase expression vector harboring mini-Tn7 with Gentr cassette; Ampr Gentr | 65 |
| pZR42 | pUC18T-mini-Tn7T-Gm with E. coli gusA; Ampr Gentr | This study |
a
Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Kanr, kanamycin resistant; Tmpr, trimethoprim resistant; Tetr, tetracycline resistant; Gentr, gentamicin resistant; Specr, spectinomycin resistant.