TABLE 1.

Gene composition of the pyocin operon in P. aeruginosa strain PA14

Gene	PA14 locus tag	Identity with HK022 homolog (%)a	Protein function
pyoRF1	PA14_07950		Activator (PrtN)
pyoRF2	PA14_07960		Repressor (PrtR)
pyoRF3	PA14_07970		Zinc finger transcription factorb
pyoRF4	PA14_07980		Lambda gpQ-likeb
pyoRF5	PA14_07990		Holinb
pyoR1	PA14_08000		Tail terminatorb
pyoR2	PA14_08010		Tail spike
pyoR3	PA14_08020		Baseplate wedge 1c
pyoR4	PA14_08030		Baseplate wedge 2c
pyoR5	PA14_08040		Baseplate wedge 3c
pyoR6	PA14_08050		Tail fiber
pyoR7	PA14_08060		Tail fiber chaperone
pyoR8	PA14_08070		Tail sheath
pyoR9	PA14_08090		Tail tube
pyoR10	PA14_08100		Tape measure chaperone
pyoR11	PA14_08110		Tape measure chaperoned
pyoR12	PA14_08120		Tape measure
pyoR13	PA14_08130		Baseplate hub 1c
pyoR14	PA14_08140		Baseplate LysM domain
pyoR15	PA14_08150		Baseplate hub 2c
pyoRF6	PA14_08160		Peptidoglycan hydrolase
pyoRF7	PA14_08180		Spannin (Rz)b
pyoRF8	PA14_08190		Spannin (Rz1)b
pyoF1	PA14_08200		Unknown
pyoF2	PA14_08210	54	Tail tube
pyoF3	PA14_08220	ND	Tape measure chaperone
pyoF4	PA14_08230	36	Tape measure chaperoned
pyoF5	PA14_08240	35	Tape measure
pyoF6	PA14_08250	37	Tail tip protein
pyoF7	PA14_08260	46	Tail tip protein
pyoF8	PA14_08270	44	Tail tip protein
pyoF9	PA14_08280	49	Tail tip protein
pyoF10	PA14_08300	44	Central fiber
pyoF11 e	NAf	24	Module 1 specificity determinant
pyoF12 e	NA	ND	Module 1 specificity determinant
pyoF13	PA14_08310	20	Module 2 specificity determinant
pyoF14	PA14_08320		Module 2 specificity determinant
pyoF15	PA14_08330		Module 2 specificity determinant

^aValues indicate the pairwise percent amino acid identity and are shown for tail morphogenesis proteins that are present in both E. coli phage HK022 and the F-type pyocin cluster. The genes encoding these proteins are arranged syntenically in the two clusters.

^bFunctions were predicted using HHpred.

^cThese myophage baseplate functions are defined by Buttner et al. (51).

^dThe second tape measure chaperone encoding segment is appended to the first through a programmed translational frameshift (52). The annotation of this ORF is missed in many P. aeruginosa genomes.

^eThese proteins, though clearly encoded in the PA14 genome, were not annotated.

^fNA, not applicable; ND, likely homologous proteins were present, but the pairwise sequence identity was below 20%.