TABLE 2.
Plasmid list
| Plasmid | Characteristics | Source |
|---|---|---|
| pEPSA5 | Shuttle vector p15A ori in E. coli, bla (Ampr) in E. coli, cat (Cmr) in S. aureus | Prahathees Eswara |
| pEP-sigS | sigS gene cloned into the EcoRI/BamHI site of pEPSA5, bla (Ampr) in E. coli, cat (Cmr) in S. aureus | This work |
| pEP-sroA | sroA gene cloned into the EcoRI/BamHI site of pEPSA5, bla (Ampr) in E. coli, cat (Cmr) in S. aureus | This work |
| pEP-sroA-FLAG | sroA-FLAG gene cloned into the EcoRI/BamHI site of pEPSA5, bla (Ampr) in E. coli, cat (Cmr) in S. aureus | This work |
| pEP-sroA-FLAGSTOP | TGA-ATG start codon substitution in sroA-FLAG gene cloned into the EcoRI/PstI site of pEPSA5; bla (Ampr) in E. coli, cat (Cmr) in S. aureus | This work |
| pEP-sroB | sroB gene cloned into the EcoRI/BamHI site of pEPSA5, bla (Ampr) in E. coli, cat (Cmr) in S. aureus | This work |
| pEP-sroAB | sroAB gene cloned into the EcoRI/BamHI site of pEPSA5; bla (Ampr) in E. coli, cat (Cmr) in S. aureus | This work |
| pEB354 | Bacterial two-hybrid interaction expression vector, PLac, LacO site, CAP binding site, p15A ori, Cyaa T25 domain linker (Kanr) | Aureilia Battesti |
| pEB354-zip | zip domain gene cloned into the EcoRI/XhoI site of Cya in T25 domain linker of pEB354 (Kanr), T25-zip | Aureilia Battesti |
| pEB354-sroA | sroA domain gene cloned into the EcoRI/XhoI site of Cya in T25 domain linker of pEB354 (Kanr), T25-sroA | This work |
| pEB354-sroB | sroB domain gene cloned into the EcoRI/XhoI site of Cya in the T25 domain linker of pEB354 (Kanr), T25-sroB | This work |
| pEB355 | Bacterial two-hybrid interaction expression vector, PLac, LacO site, CAP binding site, ColE1 ori, Cya T18 domain linker, bla (Ampr) | Aureilia Battesti |
| pEB355-zip | zip domain gene cloned into the EcoRI/XhoI site of Cya in T18 domain linker of pEB355, bla (Ampr), T18-zip | Aureilia Battesti |
| pEB355-sroA | sroA domain gene cloned into the EcoRI/XhoI site of Cya in T18 domain linker of pEB355, PLac, bla (Ampr), “T18-sroA” | This work |
| pEB355-sroB | sroB domain gene cloned into the EcoRI/XhoI site of Cya in T18 domain linker of pEB355, PLac, bla (Ampr), T18-sroB | This work |
| pIMAY* | Allelic exchange plasmid, PheS* counterselection, cat (Cmr) in S. aureus and E. coli, temp-sensitive ori (orits) in S. aureus | pIMAY* was a gift from Angelika Grundling (Addgene plasmid number 121441; https://www.addgene.org/121441/; RRID Addgene_121441) |
| pIMAY*-ΔsroAB::ermC | ΔsroAB::ermC ± 1,000 bp PCR product cloned into EcoRI/XhoI site, cat (Cmr) | This work |
a
Cya, adenylate cylase.