Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 29;14:2499. doi: 10.1038/s41467-023-38199-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Using CRISPR-Cas9 to create the ATPase-dead Ddx43 knock-in mice that carry a missense mutation (DEAD to DQAD) in the ATPase motif. Green: sgRNA targeting sequences; blue: mutated sequences; red: PAM nucleotides. b H&E staining on epididymis sections from adult Ddx43^KI/KI mice. Ddx43^KI/KI epididymis is void of normal spermatozoa that abounds in Ddx43^+/+ (Fig.1m), similar to Ddx43^–/– (Fig.1n). Scale bars are indicated. c, d Comparative analyses of body weight (c) and testis weight (d) between adult Ddx43^+/+, Ddx43^-/-, and Ddx43^KI/KI mice. N = 5 for each genotype. P values were calculated by oneway ANOVA. ns, not significant; P = 0.9621 (c; Ddx43^+/+ vs Ddx43^–/–), P = 0.6938 (c; Ddx43^+/+ vs Ddx43^KI/KI), ***P = 0.0007 (d; Ddx43^+/+ vs Ddx43^–/–), ***P = 0.0003 (d; Ddx43^+/+ vs Ddx43^KI/KI). Each bar represents the mean ± SD from biological replicates. e Fertility test on adult Ddx43^+/+, Ddx43^KI/+, and Ddx43^KI/KI mice naturally crossed with age-matched wild-type female mice. N = 5 for each genotype. P values were calculated by oneway ANOVA.ns, not significant; P = 0.8594 (Ddx43^+/+ vs Ddx43^KI/+), ***P = 0.0006 (Ddx43^+/+ vs Ddx43^KI/KI). Each bar represents the mean ± SD from biological replicates. f PAS-hematoxylin staining of testis sections from adult Ddx43^+/+, Ddx43^KI/KI and Ddx43^–/– mice. Stages of seminiferous tubule are denoted by Roman numerals. A, type A spermatogonia; IN, intermediate spermatogonia; B, type B spermatogonia; PL, preleptotene spermatocyte; L, leptotene spermatocyte; ZY, zygotene spermatocyte; PS, pachytene spermatocyte; M, metaphase cell; RS, round spermatid; ES, elongating spermatid. Red arrows point to abnormal spermatids at step 16. Scale bars are indicated. g Immunofluorescence staining of SYCP3 (green) and PNA (red) for observation of abnormal spermatids at specific stages of spermatogenesis, parallel to (f). DNA was counterstained with DAPI. Scale bars are indicated. h Representative images of transmission electron microscopy of sperm heads from adult wild-type and mutant mice. Scale bars are indicated. Each experiment was repeated three times with similar results.