(A)–(B) Quantification of colony formation of E. coli expressing a bNACHT gene with the indicated genotype on one plasmid and the indicated phage T5 gene(s) on a second plasmid. Data represent the mean ± s.e.m. of n = 3 biological replicates, shown as individual points.
(C) Visualization of plasmid integrity in E. coli expressing a bNACHT gene with the indicated genotype on one plasmid and the indicated phage T5 gene on a second plasmid. For A–C, expression of orf008, orf015, and sfGFP was induced with IPTG. Symbols denote induction of an sfGFP negative control gene (−) or induction of the indicated phage gene (+). Expression of the indicated bNACHT gene or empty vector (EV) was arabinose-inducible. Data are an image representative of n = 3 biological replicates.