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. Author manuscript; available in PMC: 2024 May 25.
Published in final edited form as: Cell. 2023 May 8;186(11):2410–2424.e18. doi: 10.1016/j.cell.2023.04.015

Figure 7. Phage proteins alter the activity of hyperactive bacterial NACHT proteins.

Figure 7.

(A)–(B) Quantification of colony formation of E. coli expressing a bNACHT gene with the indicated genotype on one plasmid and the indicated phage T5 gene(s) on a second plasmid. Data represent the mean ± s.e.m. of n = 3 biological replicates, shown as individual points.

(C) Visualization of plasmid integrity in E. coli expressing a bNACHT gene with the indicated genotype on one plasmid and the indicated phage T5 gene on a second plasmid. For A–C, expression of orf008, orf015, and sfGFP was induced with IPTG. Symbols denote induction of an sfGFP negative control gene (−) or induction of the indicated phage gene (+). Expression of the indicated bNACHT gene or empty vector (EV) was arabinose-inducible. Data are an image representative of n = 3 biological replicates.