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. 2023 Jun 17;12(6):875. doi: 10.3390/biology12060875

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cys-activated H₂Se donors (modified from ref. [109]). (A) Comparison of H₂Se release from selenocyclopropenone and selenoamides activated with Cys at pH 7.4. H₂Se can be further oxidized or trapped for detection. (B) Quantitative detection of H₂Se: reactions of Cy7-CI and different nucleophiles (H₂Se, R-SeH or R-SH).